CALLUS FORMATION FROM PROTOPLAST CULTURES OF ALLIUM WAKEGI

Protoplasts were enzymatically isolated from leaves obtained from bulb cultures of amphidiploid A. wakegi, and protoplasts were inoculated in KM8P liquid medium containing 0.1M Mannitol, 0.4m glucose. 0.4mg/12,4-D, 2 mg/1NAA and 1 mg/1BAP, and incubated in dark. Colony formation was observed within two weeks of inoculation, and developed into mini-callus after one month. The minicallus obtained were transferred to MS medium containing 3% sucrose, 0.4 mg/1 2,4-D, 2 mg/1 NAA. 1 mg/1 BAP, and 0.8% agarose, and developed into friable callus.