The Influence of The Isolated Enterobacter spp and Pantoea sp., on Barley’s Phosphorus Uptake Grown in Calcareous Soil

The purpose of this study was to determine the possibility of three phosphate-releasing bacteria (PSB) increasing the bioavailability of phosphorus (P) in a calcareous soil fertilized with single superphosphate (SSP) and its absorption by the barley (Hordeum vulgare L.) variety Giza 123. The pot experiment, a trial using calcareous soil, was conducted at the experimental farm located in Borg Al-Arab. The inoculated and uninoculated barley seeds were sown on December 1st, 2020 for 60 days. The bacterial isolates were Enterobacter aerogenes(ENPSB 1), Pantoea sp. (ENPSB 2), Enterobacter sp < /em>. (ENPSB 3), and their mixture in the ratio (1:1:1) was tested in combination with four levels of single super phosphate SSP (0%, 50%, 75%, and 100% of the recommended dosage for barley (150 kg/Fed.). In a randomized full-block design, each treatment included three replicates. Treatments and their interactions significantly affected the plant's dry weight, and bioavailable P in the soil. When these PSB strains were inoculated together (in a mixed culture), they were able to operate synergistically and were responsible for an increase in plant growth, P absorption, and accessible P in the soil when compared to a single inoculation. Also, plants inoculated with the different PSB isolates had a significant impact on total amino acids as compared with un-inoculated plants. Accordingly, we can reduce SSP application to 75% of the recommended dosage + inoculation with a mixed culture of these PSB to have a major impact on barley growth more than the application of 100% SSP of the recommended dosage without PSB inoculation to maintain environmental and soil health.